rat anticanine cd8 mab  (Bio-Rad)

Journal: Acta Pharmaceutica Sinica. B

Article Title: Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

doi: 10.1016/j.apsb.2023.11.032

Figure Lengend Snippet: The immune activation effect of OAP2 in vivo . (A–B) Analysis of mature DC cells in mouse spleen. Expression of CD80 and CD86 quantitatively detected by flow cytometry (A), and statistical analysis (B). (C–D) Analysis of mature DC cells in mouse tumor site. Expression of CD80 and CD86 quantitatively detected by flow cytometry (C), and statistical analysis (D). (E–F) Analysis of cytotoxic T cells in mouse tumor. Expression of CD4 and CD8 quantitatively detected by flow cytometry (E), and statistical analysis (F). (G–N) Tumor tissue cytokine expression content measured by ELISA ( n = 3).

Article Snippet: CD8 α (2.43) Rat mAb (PE Conjugate), CD16/CD32 (2.4G2) Rat mAb, and 7-AAD Cell Staining Solution were purchased from Cell Signaling Technology (CST).

Techniques: Activation Assay, In Vivo, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Acta Pharmaceutica Sinica. B

Article Title: Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

doi: 10.1016/j.apsb.2023.11.032

Figure Lengend Snippet: Anti-tumor metastatic effects of OAP2 in vivo . (A) Schematic representation of the OAP2 anti-tumor efficacy on 4T1 lung metastasis model; (B) Statistical analysis of tumor volume of mice during the experiment ( n = 5); (C) Representative pictures of mice at the end of treatment and H&E staining of lung; (D) Representative pictures of mice during treatment of bilateral tumor models; (E–F) Statistical analysis of tumor volume of primary (E) and distant (F) tumors in the bilateral tumor model during the experiment ( n = 5); (G) Changes of mouse body weight during the experiment of 4T1 bilateral tumor model ( n = 5); (H) Representative picture of CD8 staining of tumor tissues. Scale bars = 50 μm.

Article Snippet: CD8 α (2.43) Rat mAb (PE Conjugate), CD16/CD32 (2.4G2) Rat mAb, and 7-AAD Cell Staining Solution were purchased from Cell Signaling Technology (CST).

Techniques: In Vivo, Staining

Journal: mAbs

Article Title: Development and pharmacokinetic assessment of a fully canine anti-PD-1 monoclonal antibody for comparative translational research in dogs with spontaneous tumors.

doi: 10.1080/19420862.2023.2287250

Figure Lengend Snippet: Figure 7. Full-length IgGD P3C6mut3.1 reverses the effects of PD-L1 inhibition on canine CAR-T cells. A. K562 cells were engineered to express canine CD20 (K562- cCD20) and canine PD-L1 (K562-cCD20-cPD-L1) and cell surface expression was confirmed by flow cytometry. B. Canine CD20 CAR-T cells from one dog were labeled with cell trace violet (CTV) and co-cultured at an E:T ratio of 1:1 with either K562-cCD20 or K562-cCD20-cPD-L1 in the presence of either anti-MERS or P3C6mut3.1 IgGD. After 72 hours of culture, proliferation of CD8+ CAR-T cells was assessed by flow cytometry. Plots are gated on live CD5+ CD8+ CAR+ cells. C. Canine CD20 CAR-T cells from one dog were co-cultured at an E:T ratio of 1:1 with the same target cells as in A. Expression of CD107b was determined after 4 hours of co-culture. Plots are gated on live CD5+ CD8+ CAR+ cells.

Article Snippet: Cells were washed again twice in FACS buffer and labeled with rat anti-canine CD5 antibody (Clone: YKIX 322.3), rat anti-canine CD4 (Clone: YKIX302.9), rat anticanine CD8 mAb (Clone: YCATE55.9, BioRad, MCA1039GA), and APC-Cy7-conjugated streptavidin (BD Biosciences 554,063) for 30 min at room temperature.

Techniques: Inhibition, Expressing, Flow Cytometry, Labeling, Cell Culture, Co-Culture Assay